Journal: Stem Cells Translational Medicine
Article Title: Progranulin enhances the engraftment of transplanted human iPS cell-derived cerebral neurons
doi: 10.1093/stcltm/szae066
Figure Lengend Snippet: Gene expression analysis to identify candidate genes encoding novel host environment factors supporting grafted neurons. (A) Schematic flowchart describing the selection process of candidate genes. (B-H) Gene expression levels of the 7 candidate genes selected, as represented by count per million, for (B) Apod , (C) Ctsd , (D) Ctss , (E) Lyz2 , (F) Spp1 , (G) Grn , and (H) Sparc . Data are represented as the mean ± SEM, n = 4, Mann-Whitney test; * P < .05.
Article Snippet: The concentrations of each recombinant human protein were as follows: apolipoprotein D, APOD (NBP1-99548; Novus Biologicals, Centennial, CO, USA), 250, 500 mg/dL; cathepsin D, Ctsd (1014-AS-010; R&D Systems, Minneapolis, MN, USA), 250, 500 mg/dL; cathepsin S, Ctss (1183-CY-010; R&D Systems, Minneapolis, MN, USA), 250, 500 mg/dL; lysozyme, LYZ (ab158839; abcam, Cambridge, UK), 250, 500 mg/dL; osteopontin, OPN (1433-OP-050 CF; R&D Systems, Minneapolis, MN, USA), 250, 500 mg/dL, progranulin, PGRN (2420-PG-050; R&D Systems, Minneapolis, MN, USA), 5, 10 μg/mL; secreted protein acidic and cysteine rich, SPARC (941-SP-050; R&D Systems, Minneapolis, MN, USA), 500, 1000 mg/dL.
Techniques: Expressing, Selection, MANN-WHITNEY